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hPSC Differentiation Media
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  • hPSC Differentiation Media

hPSC Differentiation Media

Hema101 is an Animal Component-Free, Chemically Defined medium for the differentiation of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). It is suitable for trophoblast and nontrophoblast pluripotent stem cell culture systems, and the corresponding inducers need to be added when using, which can support the directional differentiation of hESCs or hiPSCs into various cell types of endoderm, mesoderm and ectoderm such as hematopoietic progenitor cells.
Features Serum-Free, Animal Component-Free, Chemically Defined
Product Type Liquid
Pack Size 100mL 500mL
Apply For Free Trial
DetailOrder InformationDocuments


Product Name

Application

Hema101

Support the directional differentiation of hESCs or hiPSCs into various cell types of endoderm, mesoderm and ectoderm such as hematopoietic progenitor cells.


Key Advantages


1. Animal Component-Free, Chemically Defined, stable product performance


2. Good compatibility, suitable for culture systems with and without trophoblasts


3. Flexible differentiation, hESC/hiPSC can be targeted into target cells according to different inducers


4. Using classical hematopoietic cell differentiation methods, 58%-84% of hematopoietic progenitor cells can be obtained



The efficiency of iHPC induction in Hema101 differentiation medium is comparable to that of similar imported media


hPSC.png

The figure shows the mean value of three independent induction differentiation experiments using hESCs, the proportion of CD45+CD34+CD43+ iHPCs in viable cells at day 16 (%), n=3, Mann-Whitney U test, P>0.05

Product Name

Cat. No.

Form

Pack Size

Hema101

K1001

Liquid

100mL   500mL


1. Product Overview

Hema101 is an animalcomponent-free, chemically defined medium for differentiation studies usinghuman embryonic stem cells (hESCs) and human induced pluripotent stem cells(hiPSCs). It is suitable for feeder-dependent or feeder-free pluripotent stemcell culture systems. With the appropriate differentiation inducers added atthe time of use, it supports directed differentiation of hESCs or hiPSCs into arange of endodermal, mesodermal, and ectodermal cell types, includinghematopoietic progenitor cells.[TR1]

2. Ordering Information

Table 1. Ordering Information


Cat. No.

Pack Size

Hema101

K1001

100mL

500mL

3. Product Specifications

Table 2. Hema101 MediumSpecifications

Physicochemical Properties

Appearance

Clear orange-red liquid

pH

6.0 - 8.0

Osmolality

275 – 325 mOsm/kg

Endotoxin

<=1.0 EU/mL

Sterile

Pass

Cell Culture Properties

Applicable Cell Line

hESC、hiPSC

Intended Use

Hematopoietic and other differentiation of pluripotent stem  cells

Shelf Life

12 months

4. Intended Use

For research and industrialproduction use only. Not for use in humans.

5. Medium Storage

Store at -80°C ±10°C. Transport at0-30°C.

6. Using the Medium

6.1 Thaw the medium at roomtemperature or 2-8°C. After thawing, store at 2-8°C and use within two weeks.Depending on the required volume, divide the thawed medium into small aliquotsand store at -80°C ±10°C until the expiration date stated on the productpackaging. Avoid additional freeze-thaw cycles.

6.2 Hema101 can be used as a basalmedium for differentiating human pluripotent stem cells (hESCs/hiPSCs) intohematopoietic progenitor cells. Published approaches include simplified 3Dmethods , multistep 3D methods, simplified 2Dmethods , and multistep 2Dmethods . Users may select andoptimize a method to suit their requirements. Hema101 can also be used fordifferentiation into other cell types, including cardiomyocytes.